Environmental reservoirs of multidrug-resistant pseudomonads in a geographical location in Kenya with high community-acquired infections

• DOI: 10.12688/f1000research.147914.1
• Publication Date: 2024-05-13T11:25:12Z
• Authors: Polly Mubassu; Abednego Musyoki; Erick Odoyo; Collins Kigen; Lillian Musila
• Keywords: Tigecycline; Colistin; Cefepime

<ns3:p>Background Pseudomonads are gram negative bacteria and readily form biofilms in the environment, allowing long-term colonization persistence sinks, water systems. They pose a risk of life-threatening opportunistic infections immune-compromised individuals. MDR strains, make treatment increasingly difficult. Environmentally persistent strains typically problematic within healthcare facilities, however, data on pseudomonad reservoirs settings with community-acquired to inform preventive interventions, resource-constrained is scarce. Here, we determined antibiotic susceptibility <ns3:italic>Pseudomonas</ns3:italic> species sources Kisumu County, Kenya reported high levels community acquired infections. Methods We adopted cross-sectional design, randomly collecting 297 samples from tap heads, tanks, vendor household storage containers six selected sub-locations one hospital (KCRH)<ns3:italic>.</ns3:italic> Standard microbiological procedures were used for identification AST isolates. Results isolated <ns3:italic>Pseudomonads</ns3:italic> 14.1% collected, predominantly 10.4%. Seven different pseudomonads identified, <ns3:italic>Pseudomonas aeruginosa</ns3:italic> predominating 6.7% overall, 5.7%, among isolates tanks 21.4%. Pseudomonad 62% non-susceptible piperacillin, 57% tigecycline, 24% meropenem, 21% cefepime, 19% levofloxacin 14% colistin. Carbapenem resistance was mainly detected <ns3:italic>P. 80% (8/10) Milimani sub-location 75% (6/8). 45% recovered MDR, community-associated carbapenem-resistant (CRPA) 42%, susceptible The exhibited multiple indices, ranging 0.43 1. Conclusion This study reveals higher prevalence pseudomonads, including CRPA sources. These potential conduits drug present critical public health threat, especially immunocompromised. Regular cleaning implementation antimicrobial stewardship programs, required prevent rise AMR eliminate environmental that put vulnerable populations at risk.</ns3:p>