Absence of toll-like receptor 9 Pro99Leu polymorphism in cervical cancer

• DOI: 10.12688/f1000research.14840.2
• Publication Date: 2018-08-30T11:55:54Z
• Authors: Alex Chauhan; Nilesh Pandey; Nitin Raithatha; Purvi Patel; Ajesh Desai; Neeraj Jain
• Keywords: Adult; 0301 basic medicine; 0303 health sciences; Genotype; India; Uterine Cervical Neoplasms; Middle Aged; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; White People; 3. Good health; 03 medical and health sciences; Gene Frequency; Case-Control Studies; Toll-Like Receptor 9; Humans; Female; Genetic Predisposition to Disease; Polymorphism, Restriction Fragment Length; Research Article

Background: Toll-like receptor 9 (TLR9) plays a key role in the elimination of viral pathogens by recognising their CpG DNA. Polymorphisms in the TLR9 gene may influence their recognition and subsequent elimination. Therefore, the present study was designed to elucidate the role of a rare unexplored TLR9 gene polymorphism C296T/ Pro99Leu (rs5743844) in cervical cancer susceptibility among Indian women. Methods: The genotyping of TLR9 Pro99Leu polymorphism in 110 cervical cancer patients and 141 healthy controls was performed by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Results: The genotype frequency detected in both cervical cancer and control populations was 1.0 (CC), 0.0 (CT) and 0.0 (TT); while the allele frequency was found to be 1.0 (C) and 0.0 (T). Conclusions: The present study demonstrates no involvement of TLR9 C296T/ Pro99Leu polymorphism in cervical cancer susceptibility and supports minor allele frequency (MAF) (0.0002) status of the same as no nucleotide variation was detected in any of the study subjects.