Antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro oral biofilm

• DOI: 10.12688/f1000research.73602.1
• Publication Date: 2021-10-27T16:40:06Z
• Authors: Pablo Alejandro M...; Lidia Yileng Tay ...; Dora Jesús Maurtu...; Reyma Evelyn Baci...; Ingrit Elida Coll...; Carlos Alberto Mi...
• Keywords: Propolis; Streptococcus gordonii

<ns3:p><ns3:bold>Background:</ns3:bold> Natural products with antibacterial potential have begun to be tested on biofilm models, bringing us closer understanding the response generated by complex microbial ecosystems of oral cavity. The objective this study was evaluate antibacterial, antibiofilm, and cytotoxic activities chemical compositions Peruvian propolis in an <ns3:italic>in vitro</ns3:italic> <ns3:italic>Streptococcus gordonii </ns3:italic>and<ns3:italic> Fusobacterium nucleatum</ns3:italic>.</ns3:p><ns3:p> <ns3:bold>Methods:</ns3:bold> experimental work involved a consecutive, vitro</ns3:italic>, longitudinal, double-blinded design. Propolis samples were collected from 13 different regions Andes. disk diffusion method used for antimicrobial susceptibility test. effect human gingival fibroblasts determined cell viability using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, evaluated confocal microscopy polymerase chain reaction (PCR).</ns3:p><ns3:p> <ns3:bold>Results:</ns3:bold> 0.78 mg/mL 1.563 concentrations methanolic fraction chloroform residue Oxapampa showed effects thickness copy numbers <ns3:italic>srtA</ns3:italic> gene <ns3:italic>S. gordonii</ns3:italic> <ns3:italic>radD</ns3:italic> <ns3:italic>F. nucleatum</ns3:italic> at 48 120 hours, chromatography (UV, λ 280 nm) identified rhamnocitrin, isorhamnetin, apigenin, kaempferol, diosmetin, acacetin, glycerol, chrysoeriol.</ns3:p><ns3:p> <ns3:bold>Conclusions: </ns3:bold>Of evaluated, it found that only antibiofilm without causing damage fibroblasts. Likewise, when evaluating composition fraction, eight flavonoids identified.</ns3:p>