Optimization of extraction of genomic DNA from archived dried blood spot (DBS): potential application in epidemiological research & bio banking

• DOI: 10.12688/gatesopenres.12855.3
• Publication Date: 2019-11-14T12:15:32Z
• Authors: Abhinendra Kumar; Sharayu Mhatre; Sheela Godbole; Prabhat Jha; Rajesh Dikshit
• Keywords: genomic DNA

<ns4:p><ns4:bold>Background:</ns4:bold>Limited infrastructure is available to collect, store and transport venous blood in field epidemiological studies. Dried spot (DBS) a robust potential alternative sample source for studies &amp; bio banking. A stable of genomic DNA (gDNA) required long term storage bank its downstream applications. Our objective optimize the methods gDNA extraction from stored DBS with aim revealing utility large scale </ns4:p><ns4:p><ns4:bold>Methods:</ns4:bold>The purpose this study was extract maximum amount on Whatman 903 protein saver card. extracted through column (Qiagen) magnetic bead based (Invitrogen) methods. Quantification performed spectrophotometer, fluorometer, integrity analyzed by agarose gel electrophoresis. </ns4:p><ns4:p><ns4:bold>Result:</ns4:bold>Large variation observed quantity purity (260/280 ratio, 1.8-2.9) gDNA. The intact bands electrophoresis reflect robustness even after prolonged time. 2.16 – 24 ng/µl sufficient PCR application, but unfortunately it can’t be used whole genome sequencing or genotyping Sequencing can achieved increasing template copy number amplification obtained results create base future research develop high-throughput samples.</ns4:p><ns4:p><ns4:bold>Conclusion:</ns4:bold>The above reveal, utilized as banking studies.</ns4:p>