Punica Granatum Peel Extract Protects Against Ionizing Radiation-Induced Enteritis And Leukocyte Apoptosis In Rats

• DOI: 10.1269/jrr.08126
• Publication Date: 2009-07-31T05:57:31Z
• Authors: Hale Z. TOKLU; Özer ŞEHİRLİ; Hazan ÖZYURT; A. Alpaslan MAYAD...; Emel EKŞİOĞLU-DEM&#; Şule ÇETİNEL; Hülya ŞAHİN; Berrak Ç. YEĞEN; Melek ULUSOYLU DUMLU; Vural GÖKMEN; Göksel ŞENER
• Keywords: Malondialdehyde; Radiation Enteritis

Radiation-induced enteritis is a well-recognized sequel of therapeutic irradiation. Therefore we examined the radioprotective properties Punica granatum peel extract (PPE) on oxidative damage in ileum. Rats were exposed to single whole-body X-ray irradiation 800 cGy. Irradiated rats pretreated orally with saline or PPE (50 mg/kg/day) for 10 days before and following days, while control received but no Then plasma ileum samples obtained. Irradiation caused decrease glutathione total antioxidant capacity, which was accompanied by increases malondialdehyde levels, myeloperoxidase activity, collagen content tissue concomitant increase 8-hydroxy-2'-deoxyguanosine (an index DNA damage). Similarly, pro-inflammatory cytokines (TNF-alpha, IL-1beta IL-6) lactate dehydrogenase elevated irradiated groups as compared control. treatment reversed all these biochemical indices, well histopathological alterations induced Furthermore, flow cytometric measurements revealed that leukocyte apoptosis cell death increased animals, effects. supplementation reduced ileal tissues, probably mechanism associated decreased production reactive oxygen metabolites enhancement mechanisms. Adjuvant therapy may have potential support successful radiotherapy protecting against radiation-induced enteritis.