Benzo[a]pyrene Cytotoxicity Tolerance in Testicular Sertoli Cells Involves Aryl-hydrocarbon Receptor and Cytochrome P450 1A1 Expression Deficiencies

• DOI: 10.12717/dr.2021.25.1.15
• Publication Date: 2021-04-16T11:33:31Z
• Authors: Jin-Tac Kim; Ji-Eun Park; Seung-Jin Lee; Wook-Joon Yu; Hye-Jeong Lee; Jong-Min Kim
• Keywords: 0301 basic medicine; 03 medical and health sciences; Research; 3. Good health

Benzo[a]pyrene (B[a]P) is a potent carcinogen and classified as an endocrine-disrupting chemical. In mammalian testes, Sertoli cells support spermatogenesis. Therefore, if these are negatively affected by exposure to xenotoxic chemicals, spermatogenesis can be seriously disrupted. this context, we evaluated whether mouse testicular TM4 susceptible the induction of cytotoxicity-mediated cell death after B[a] P in vitro. present study, while B[a]P B[a]P-7,8-diol were not able induce death, BPDE resulted death. BPDE-induced accompanied activation caspase-3 caspase-7. Depolarization mitochondrial membrane cytochrome c release from mitochondria observed benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE)-treated cells. These results indicate that apoptosis caspase-dependent manner. Western blot reverse transcription-polymerase chain reaction (RT-PCR) analyses showed aryl hydrocarbon receptor (AhR) expression was almost undetectable its altered treatment. This indicates nearly AhR-deficient. cells, CYP1A1 protein activity present. From results, it clear AhR may prerequisite for referred CYP1A1-deficient Thus, believed have rigid protective cellular machinery against genotoxic agents. conclusion, suggested tolerance cytotoxicity associated with insufficient