A Newcastle disease live virus vaccine is safe and efficacious at various storage conditions.

• DOI: 10.12834/vetit.2980.19377.1
• Publication Date: 2024-09-18
• Authors: Iffat Huma; Shahan Azeem; Sarwat Naz; Tahir Yaqub; Muhammad Hassan M...; Muhammad Rizwan Azam
• Keywords: Newcastle Disease

Pure, potent and efficacious vaccines could help in the control of Newcastle disease (ND). The present study was designed to evaluate thermo-stability a live-attenuated ND virus vaccine containing Mukteswar strain genetically characterize seed virus. Moreover, presence extraneous agents (Fowl adenovirus, Mycoplasma, Salmonella Pullorum, Gallinarum) assessed using polymerase chain reactions (PCR) optimized for detection panel. evaluated its potency efficacy after storage at 4°C, 25°C 37°C 36, 48, 96 144 hours. A total 100 commercial broiler chickens were randomly divided into six groups immunized with stored specified temperatures given times. Blood samples collected on days 0, 7, 14, 21 28 post-vaccination, sera separated antibody titers hemagglutination inhibition (HI) assay. data analyzed by two-way analysis variance (ANOVA) multivariate (MANOVA). Reverse-transcription PCR targeting F gene (NDV) subsequent sequence confirmed NDV (deposited GenBank Acc. Nos. MK310260 MK310261). Phylogenetic revealed close resemblance other Avian Avulaviruses (NDV class II Genotype III viruses more specifically strains), yet it distinct from Pakistani field isolates, which grouped genotype XIII.2.1. testing that free agents. study's findings propose an alternative rapid PCR-based method purity live vaccines. Together these suggest tested is pure, efficacious, continuous maintenance cold recommended maintain efficacy.