Isolation, identification and characterization of novel <i>Bacillus subtilis</i>

• DOI: 10.1292/jvms.16-0572
• Publication Date: 2018-01-22T22:26:33Z
• Authors: Zhenxiang LU; Weina GUO; Chang LIU
• Keywords: Enterococcus faecalis; Bacillus (shape)

In this study, we have identified a bacterium that can inhibit the growth of Staphylococcus aureus, and further analyzed its antibacterial activity other biological characteristics laid foundation for future application. Through isolation culture unknown bacteria, characteristics, morphology observation, biochemical test, preliminary 16S rRNA PCR amplification, sequence analysis, homology analysis were performed. It was found bacteria are Gram positive spore chain Bacillus. The could only ferment glucose acid production, but not utilize lactose maltose. VP test positive, while indole methyl red tests negative. Further showed these shared up to 99.4% with Bacillus subtilis DQ198162.1. Thus, newly classified as subtilis. Importantly, crude bacteriocin Escherichia coli, Enterococcus Salmonella, which implies potential usage in future.