A Comparison of the In Vivo and In Vitro Response of Rat Embryos to 5-Fluorouracil.

• DOI: 10.1292/jvms.60.93
• Publication Date: 2002-10-09T03:03:37Z
• Authors: Makiko KUWAGATA; Hiromasa TAKASHIMA; Tetsuji NAGAO
• Keywords: 0301 basic medicine; Fetal Growth Retardation; Pregnancy Outcome; Abnormalities, Drug-Induced; Anophthalmos; Gestational Age; Embryo, Mammalian; Rats; 3. Good health; Rats, Sprague-Dawley; Embryonic and Fetal Development; 03 medical and health sciences; Organ Culture Techniques; Pregnancy; Animals; Female; Fluorouracil

This study serves to further define the capabilities of whole embryo culture system using well-known teratogen, 5-fluorouracil (5-FU), an antineoplastic agent. An initial in vivo was performed whereby pregnant rats were injected intraperitoneally with 10-30 mg/kg 5-FU on day 9 gestation. On 20 gestation, effects this drug growth and development embryos evaluated. The number externally malformed fetuses increased a dose-related manner, most common defect micro-/anophthalmos dams treated 5-FU. Growth retardation also noted groups. vitro which concentrations varied (0.15-0.30 μg/ml). Externally abnormal observed from embryonic 11. hypoplastic optic vesicles. In system, crown-rump length, somite number, protein contents, morphological score decreased dose-dependent fashion. Finally, histological evaluation observation pattern cell death vesicle 11-day-old performed. These parameters revealed no differences response between 5-FU, suggesting that appropriate model for developmental toxicity studies