Molecular Identification, Antagonistic Assay and Enzyme Profiling of Selected Trichoderma Isolates

• DOI: 10.12944/carj.12.2.20
• Publication Date: 2024-09-20T05:55:08Z
• Authors: Utkarsh Singh Rat...; Rudra Pratap Singh; Sonika Pandey; R. K. Mishra

The aim of this study was to find and characterize Trichoderma isolates by antagonistic and enzymatic activity to evaluate their potential as biocontrol agents against Dry root rot (DRR). Trichoderma isolates were isolated from thepulses rhizosphere of different districts of Uttar Pradesh. Twenty one Trichoderma isolates were identified using ribosomal DNA internal transcribed spacer (ITS) regions and translation elongation factor 1-alpha (tef1). In addition, enzymatic profiling of Trichoderma isolates was done indicated strong cell wall degrading enzyme activities and plant growth promoting traits of Trichoderma isolates. Overall, our results suggested that the isolated Trichoderma spp. have prodigious potential for plant growth promotion and can be used as biocontrol agents against dry root rot.