Gadobutrol-dendrimer effects on metastatic and apoptotic gene expression

• DOI: 10.12989/anr.2016.4.2.145
• Publication Date: 2016-08-03T02:24:47Z
• Authors: Abbas Kebriaezadeh; Sepehr Ashrafi; Rahimeh Rasouli; Seyed Esmaeil Sad...; Morteza Pirali Ha...; Artin Assadi; Mostafa Saffari; Mehdi Shafiee Ard...
• Keywords: MTT assay

Dendrimers are one of the most appropriate nanocaries for imaging moieties in applications.The purpose this study was evalution cytotoxicity and inducing apoptosis dendrimers. This conducted order to investigate metastasis suppression effect dendrimer human breast MCF-7 cell line finding nanoparticle protein corona biological enviromental. Dendrimer assessed by MTT assay. The mRNA experession level KAI1 as a suppressor gene, Bax Pro- apoptotic Bcl-2 an anti-apoptotic gene GAPDH housekepping were determined real-time PCR assays.concentration-dependent proofed at range 1-2 mg/mL 24 hours, significant upregulation expression Bax, observed whereas down-regulated, also up-regulated. So far few studies confirmed enhancement dendrimers via bax/bcl-2 pathways. nanoparticles able act metastase inhibitor gene.