Development and validation of a reversed-phase HPLC method for the determination of lisinopril and gliclazide in pharmaceuticals

• DOI: 10.12991/mpj.72766
• Publication Date: 2017-04-01
• Authors: Sevil Şenkardeş; Tuğçe Özaydın; Timuçin Uğurlu; Ş. Güniz Küçükgüzel
• Keywords: Gliclazide; Lisinopril

The aim of the present study was to develop and validate a High-Performance Liquid Chromatography (HPLC) method for determination lisinopril gliclazide. developed on Zorbax C 8 analytical column (4,6x250 mm; 5µm) by isocratic elution with flow rate 1.0 ml/min injection volume 25 µl. mobile phase composition methanol:water (65:35 v/v, pH adjusted 3.0 triethylamine-orthophosphoric acid buffer) retention time found be 2.883 7.456 min gliclazide, respectively. linear in concentration range 5 µg/ml 20 15 60 validated linearity, accuracy, precision, LOD LOQ. This procedure applied conveniently analysis gliclazide pharmaceutical preparations. Key Words: Gliclazide, lisinopril, RP-HPLC, validation