[Distribution Characteristics and Influencing Factors of Antibiotic Resistance Genes in Surface Sediments of Regulating Pond in South-to-North Water Transfer Pumping Station].

• DOI: 10.13227/j.hjkx.202312226
• Publication Date: 2025-03-08
• Authors: Fei Li; Chunsheng Qiu; Chenchen Wang; Fei Xu; Dong Wang; Nannan Liu; Chen Xu; Shaopo Wang
• Keywords: Gene transfer; Water transfer; Horizontal Gene Transfer

As an important facility to ensure the safety of water supply, regulating pond a raw pumping station in Tianjin Section middle route South-to-North Water Transfer Project linked from Hanjiang River with urban plants. Surface sediment samples different regions regulator were collected summer, autumn, and winter 2022 spring 2023, respectively. Metagenomic sequencing technology was used analyze distribution characteristics influencing factors antibiotic resistance genes （ARGs）, correlation between ARGs physicochemical indices, microbial community structure, mobile genetic elements （MGEs） also analyzed. The results showed that 20 types 921 subtypes detected surface pond. dominant at sampling times points multidrug resistance, MLS, tetracycline, glycopeptides, main mechanism efflux pump. Correlation analysis TN, NO3--N, TP, OM sediments significantly correlated （P<0.05） various top ARGs. Among genera, 19 species MGEs conjugate transfer protein, recombinase, transposase positively