Countercurrent chromatography in analytical chemistry (IUPAC Technical Report)

• DOI: 10.1351/pac-rep-08-06-05
• Publication Date: 2009-02-23T13:44:38Z
• Authors: Alain Berthod; Tatyana Maryutina; Boris Spivakov; Oleg Shpigun; Ian A. Sutherland
• Keywords: Countercurrent exchange; Liquid phase

Abstract Countercurrent chromatography (CCC) is a generic term covering all forms of liquid-liquid that use support-free liquid stationary phase held in place by simple centrifugal or complex force field. Biphasic systems are used with one being the and other mobile phase. Although initiated almost 30 years ago, CCC lacked reliable columns. This changing now, newly designed centrifuges appearing on market make excellent review focuses advantages addresses chromatographic theory CCC. The main difference classical (LC) variable volume There mainly two different ways to obtain using forces, hydrostatic way hydrodynamic way. These kinds columns described compared. reported applications analytical chemistry comparison separation enrichment methods show technique can be successfully analysis plants natural products, for biochemicals pharmaceuticals, alkaloids from medical herbs, food analysis, etc. On basis studies last decades, recommendations also given application trace inorganic radioanalytical chemistry.