Bone Morphogenetic Proteins in Bone Stimulate Osteoclasts and Osteoblasts During Bone Development

• DOI: 10.1359/jbmr.060411
• Publication Date: 2006-07-04T05:47:20Z
• Authors: Mina Okamoto; Junko Murai; Hideki Yoshikawa; Noriyuki Tsumaki
• Keywords: Bone remodeling period; Bone remodeling; Bone development

Abstract In this study, overexpression of noggin, a BMP antagonist, in developing bone caused significantly decreased osteoclast number as well formation rate, resulting increased mass with immature quality. signaling plays important roles normal development and regulation resorption. Introduction: Bone morphogenetic proteins (BMPs) act on various types cells. Although involvement signals osteoblast differentiation has been studied extensively, the effects BMPs osteoclasts have not widely researched. Consequently, net remain unclear. The purpose study was to delineate more fully role skeletal biology. Materials Methods: We generated transgenic mice that express BMP4 or noggin under control 2.3-kb α1(I) collagen chain gene (Col1a1) promoter, analyzed their phenotype. also expressing specifically cartilage. Results: Mice overexpressing developed severe osteopenia number. showed volume associated rate noggin-transgenic tibias exhibited reduced periosteal resorption marrow spaces, frequent fractures at diaphysis. Co-culture primary osteoblasts prepared from calvariae wildtype spleen cells resulted poor formation, which rescued by addition recombinant BMP2, suggesting inhibits attenuating activities mice. expression levels Rankl were Immunoblot analysis phosphorylation Smad1/5/8 precursor after 20-minute treatment BMPs, these are stimulated BMPs. cartilage had enlarged bones containing thick trabeculae, possibly because expansion anlagen. Conclusions: Overexpression revealed regulate through stimulation osteoclasts.