Influence of hematoxylin and eosin staining on the quantitative analysis of second harmonic generation imaging of fixed tissue sections

• DOI: 10.1364/boe.428701
• Publication Date: 2021-07-20T18:00:08Z
• Authors: Radu Hristu; Stefan G. Stanciu; Adrian Dumitru; Bogdan Paun; Iustin Floroiu; Mariana Costache; George A. Stanciu
• Keywords: Eosin; Second-harmonic imaging microscopy

Second harmonic generation (SHG) microscopy has emerged over the past two decades as a powerful tool for tissue characterization and diagnostics. Its main applications in medicine are related to mapping collagen architecture of in-vivo, ex-vivo fixed tissues based on endogenous contrast. In this work we present how H&E staining excised influences extraction use image parameters specific polarization-resolved SHG (PSHG) microscopy, which known provide quantitative information structure organization. We employ theoretical model fitting experimental PSHG datasets obtain second order susceptibility tensor elements ratios efficiency. Furthermore, intensity acquired under circular polarization is investigated. The evolution these both forward- backward-collected computed H&E-stained unstained sections. Consistent modifications observed between cases terms efficiency intensity. This suggests that similar analysis workflows applied images collected stained could yield different results, hence affect diagnostic accuracy.