High-fidelity optical diffraction tomography of live organisms using iodixanol refractive index matching

• DOI: 10.1364/boe.465066
• Publication Date: 2022-11-14T17:06:31Z
• Authors: Dohyeon Lee; Moosung Lee; Haechan Kwak; Young Seo Kim; Jaehyu Shim; Jik Han Jung; Wei-sun Park; Ji-Ho Park; Sumin Lee; YongKeun Park
• Keywords: Iodixanol; Biological Imaging

Optical diffraction tomography (ODT) enables the three-dimensional (3D) refractive index (RI) reconstruction. However, when RI difference between a sample and medium increases, effects of light scattering become significant, preventing acquisition high-quality accurate reconstructions. Herein, we present method for high-fidelity ODT by introducing non-toxic matching media. Optimally reducing contrast enhances fidelity accuracy 3D reconstruction, enabling visualization morphology intra-organization live biological samples without producing toxic effects. We validate our using various organisms, including C. albicans elegans.