Motionless volumetric structured light sheet microscopy

• DOI: 10.1364/boe.489280
• Publication Date: 2023-04-12T10:00:10Z
• Authors: Tyler Peterson; Shivani Mann; Belinda L. Sun; Leilei Peng; Haijiang Cai; Rongguang Liang
• Keywords: Structured Light

To meet the increasing need for low-cost, compact imaging technology with cellular resolution, we have developed a microLED-based structured light sheet microscope three-dimensional ex vivo and in of biological tissue multiple modalities. All illumination structure is generated directly at microLED panel—which serves as source—so scanning modulation completely digital, yielding system that simpler less prone to error than previously reported methods. Volumetric images optical sectioning are thus achieved an inexpensive, form factor without any moving parts. We demonstrate unique properties general applicability our technique by porcine murine from gastrointestinal tract, kidney, brain.