Accurate detection and complete tracking of large populations of features in three dimensions

• DOI: 10.1364/oe.17.004685
• Publication Date: 2009-03-09T19:46:50Z
• Authors: Yongxiang Gao; Maria L. Kilfoil
• Keywords: Tracking (education); Particle (ecology)

Localization and tracking of colloidal particles in microscopy images generates the raw data necessary to understand both dynamics mechanical properties model systems. Yet, despite obvious importance analyzing particle movement three dimensions (3D), accurate sub-pixel localization 3D has received little attention so far. Tracking been limited by choice whether track all a low-density system, or neglect most mobile fraction dense system. Moreover, assertions are frequently made on accuracies methods for locating colloid physics biology, field can be well-served quantitative comparison relative performances. We show that iterating dimensions, centers more accurately located three-dimensions (3D) than with previous at least half an order magnitude. In addition, we implementing multi-pass deflation approach, greater fidelity achieved reconstruction trajectories, once positions known. general, future work must defend accuracy tracks considered reliable. Specifically, other researchers use presented here (or alternative whose substantianted) entire investigation legitimate, if basis physical argument (in colloids, any application) depends positions. compare our algorithms recent related advances location/tracking colloids discuss strengths weaknesses various situations. carry out performance tests directly comparing simulated location tracking, providing data, assess just how software locate particles. methods, now applied could improve features such as quantum dots cells.