High-resolution, label-free imaging of living cells with direct electron-beam-excitation-assisted optical microscopy

• DOI: 10.1364/oe.23.014561
• Publication Date: 2015-05-27T16:41:26Z
• Authors: Yasunori Nawa; Wataru Inami; Sheng Lin; Yoshimasa Kawata; Susumu Terakawa
• Keywords: Cell Nucleus; 0301 basic medicine; Microscopy; Osteoblasts; Optical Phenomena; Staining and Labeling; Cell Survival; Electrons; Cell Line; Mitochondria; Actin Cytoskeleton; Mice; 03 medical and health sciences; Imaging, Three-Dimensional; Animals

High spatial resolution microscope is desired for deep understanding of cellular functions, in order to develop medical technologies. We demonstrate high-resolution imaging of un-labelled organelles in living cells, in which live cells on a 50 nm thick silicon nitride membrane are imaged by autofluorescence excited with a focused electron beam through the membrane. Electron beam excitation enables ultrahigh spatial resolution imaging of organelles, such as mitochondria, nuclei, and various granules. Since the autofluorescence spectra represent molecular species, this microscopy allows fast and detailed investigations of cellular status in living cells.