Deformable mirror based optimal PSF engineering for 3D super-resolution imaging

• DOI: 10.1364/ol.460949
• Publication Date: 2022-05-24T11:30:44Z
• Authors: Shuang Fu; Mengfan Li; Lulu Zhou; Yingchuan He; Xin Liu; Xiang Hao; Yiming Li
• Keywords: Point spread function; Astigmatism; Deformable Mirror; Spatial light modulator

Point spread function (PSF) engineering is an important technique to encode the properties (e.g., 3D positions, color, and orientation) of a single molecule in shape PSF, often with help programmable phase modulator. A deformable mirror (DM) currently most widely used modulator for fluorescence detection as it shows negligible photon loss. However, relies on careful calibration precise wavefront control. Therefore, design optimal PSF not only theoretical calculation maximum information content, but also physical behavior modulator, which ignored during optimization process. Here, we develop framework could generate device specific super-resolution imaging using DM. We use our method two types PSFs depths field comparable astigmatism tetrapod PSFs, respectively. demonstrate superior performance DM over conventional both theoretically experimentally.