Use of coherent control for selective two-photon fluorescence microscopy in live organisms

• DOI: 10.1364/opex.14.000759
• Publication Date: 2006-02-13T17:13:34Z
• Authors: Jennifer P. Ogilvie; Delphine Débarre; Xavier Solinas; Jean-Louis Martin; Emmanuel Beaurepaire; Manuel Joffre
• Keywords: [PHYS.PHYS.PHYS-OPTICS] Physics [physics]/Physics [physics]/Optics [physics.optics]; [SDV.IB] Life Sciences [q-bio]/Bioengineering; [PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics]; [SDV.IB]Life Sciences [q-bio]/Bioengineering; 02 engineering and technology; 0210 nano-technology

We demonstrate selective fluorescence We demonstrate selective fluorescence excitation of specific molecular species in live organisms by using coherent control of two-photon excitation. We have acquired quasi-simultaneous images in live fluorescently-labeled Drosophila embryos by rapid switching between appropriate pulse shapes. Linear combinations of these images demonstrate that a high degree of fluorophore selectivity is attainable through phase-shaping. Broadband phase-shaped excitation opens up new possibilities for single-laser, multiplex, in-vivo fluorescence microscopy.