Polarization-resolved second-harmonic generation imaging through a multimode fiber

• DOI: 10.1364/optica.430295
• Publication Date: 2021-07-02T14:00:51Z
• Authors: Angel Cifuentes; Tomáš Pikálek; Petra Ondráčková; Rodrigo Amezcua-C...; José Enrique Anto...; Tomáš Čižmár; Johanna Trägårdh
• Keywords: Endoscope; Collagen fiber; Native tissue

Multimode fiber-based endoscopes have recently emerged as a tool for minimally invasive endoscopy in tissue, at depths well beyond the reach of multiphoton imaging. Here, we demonstrate label-free second-harmonic generation (SHG) microscopy through such fiber endoscope. We simultaneously fully control excitation polarization state and spatial distribution light tip, use this to implement polarization-resolved SHG imaging, which allows imaging identification structural proteins collagen myosin. image mouse tail tendon heart employing endoscope up 1 mm, demonstrating that can differentiate these proteins. This method has potential enabling instant situ diagnosis tumors fibrotic conditions sensitive tissue with minimal damage.