Human Saliva-Derived Exosomes

• DOI: 10.1369/0022155414564219
• Publication Date: 2014-12-04T02:42:25Z
• Authors: Ayelet Zlotogorsk...; Dan Dayan; Gavriel Chaushu; Johanna Korvala; Tuula Salo; Raija Sormunen; Marilena Vered
• Keywords: CD63; CD81; Immunoelectron microscopy; Exosome

ExoQuick-TC TM (EQ), a chemical-based agent designed to precipitate exosomes, was calibrated for use on saliva collected from healthy individuals. The morphological and molecular features of the precipitations were compared with those obtained using classical, physical-based method ultracentrifugation (UC). Electron microscopy immunoelectron anti-CD63 showed vesicular nanoparticles surrounded by bi-layered membrane, compatible exosomes in EQ, similar that observed UC. Atomic force highlighted larger, irregularly shaped/aggregated EQ contrasted single, round-shaped UC nanoparticles. ELISA (performed 0.5 ml saliva) revealed tendency higher expression specific exosomal markers (CD63, CD9, CD81) than ( p>0.05). epithelial growth factor receptor, non-exosomal-related marker, significantly concentration p=0.04). Western blotting equal total-protein concentrations bands CD63, CD9 CD81 both types preparations, although they less pronounced This may be related fraction non-exosomal proteins EQ. In conclusion, is suitable efficient precipitation salivary small volumes saliva; however, tends associated considerably more biological impurities (non-exosomal-related proteins/microvesicles) as