Cardiomyocyte Remodeling and Sarcomere Addition after Uniaxial Static Strain In Vitro

• DOI: 10.1369/jhc.4a6608.2005
• Publication Date: 2005-07-01T20:23:14Z
• Authors: Ji-Guo Yu; Brenda Russell
• Keywords: Myofibril; Strain (injury); Actinin; Intercalated disc

Individual cardiomyocytes are lengthened in dilated cardiomyopathy. However, it is not known how the new sarcomeres added to preexisting myofibrils. Using a three-dimensional microtextured culturing system, 10% mechanical static strain was applied aligned, well-attached from neonatal rat. The morphology of myofibrils and ends myocytes were examined. Disruptions sarcomeric pattern for actin showed progression weak intense staining over 4 hr. lightly stained common at 1 hr after being strained, peaked 2 hr, then subsided. In contrast, numbers intensely initially low, 3 began decline when compared with control values. myocyte elongations convolutions observed alpha-actinin N-cadherin staining. We suggest that rat hearts remodel by insertion throughout cell length also enhancement intercalated discs.