[Mechanism of electroacupuncture in improving cartilage tissue injury in rats with knee osteoarthritis by regulating miR-335-5p expression].

• DOI: 10.13702/j.1000-0607.20240617
• Publication Date: 2025-03-25
• Authors: Hao Zhu; Xuehua Yan; Shuai Chen; Gai-Yue Zhang; Haobin Zhang; Jinsheng Yang; Yingying Wang

To observe the protective effect of electroacupuncture (EA) on knee joint cartilage tissue rats with osteoarthritis (KOA), so as to explore potential mechanism miR-335-5p involved in treatment EA for KOA. A total 42 adult male Sprague-Dawley (SD) were randomly divided into blank, model and groups, 14 each group. The was established by injecting a sodium glutamate iodoacetate solution (80 μg/μL) right cavity. On 15th day after establishment, group received intervention at "Yanglingquan" (GB34) "Xuehai" (SP10) side, needles retained 15 minutes. carried out every other two weeks. After two-week intervention, paw withdrawal mechanical threshold (PWMT) thermal latency (PWTL) detected;a gait analyzer used detect adaptability changes induced pain;the pathological observed hematoxylin-eosin (HE) staining;qPCR Western blot expression levels miR-335-5p, Dickkopf related protein 1 (DKK-1), β-catenin, matrix metalloproteinase 13 (MMP-13) mRNAs proteins respectively;immunofluorescence staining positive fluorescence β-catenin tissue. In group, HE showed large area chondrocyte necrosis, dissolution disappearance cell nuclei, accompanied macrophages-predominant inflammatory infiltration;cartilage surface defects local areas, necrosis trabecular bone proliferation fibrous new capillary formation proliferative areas also observed. manifestations such infiltration significantly alleviated. Compared blank PWMT, PWTL, support duration, footprint area, average ground pressure hind paw, mRNA expressions DKK-1 decreased (P<0.001) while MMP-13, intensity increased (P<0.001, P<0.01). All above indicators all reversed P<0.05, P<0.01) comparison can improve pain symptoms KOA rats, promote recovery motor function, morphology This may be regulating miR-355-5p Wnt/β-catenin pathway, well inhibiting release extracellular matrix-related degrading enzymes.