Correction: Compound A, a Dissociated Glucocorticoid Receptor Modulator, Inhibits T-bet (Th1) and Induces GATA-3 (Th2) Activity in Immune Cells

• DOI: 10.1371/annotation/12a8fc89-5f47-4bad-8863-863d99a0e52d
• Publication Date: 2012-06-01T15:29:05Z
• Authors: Ana C. Liberman; Maria Antunica-No...; Viviane Ferraz-de...; Joao Palermo-Neto; Carla N. Castro; Jimena Druker; Florian Holsboer; Marcelo J. Perone; Sarah Gerlo; Karolien De Bosscher; Guy Haegeman; Eduardo Arzt
• Keywords: Proinflammatory cytokine

Background: Compound A (CpdA) is a dissociating non-steroidal glucocorticoid receptor (GR) ligand which has antiinflammatory properties exerted by down-modulating proinflammatory gene expression.By favouring GR monomer formation, CpdA does not enhance (GC) response element-driven expression, resulting in reduced side effect profile as compared to GCs.Considering the importance of Th1/Th2 balance final outcome immune and inflammatory responses, we analyzed how selective modulation differentially regulates activity T-bet GATA-3, master drivers Th1 Th2 differentiation, respectively.Results: Using Western analysis reporter assays, show murine T cells that, similar GCs, inhibits via transrepressive mechanism.Different from induces GATA-3 p38 MAPK-induction phosphorylation nuclear translocation.CpdA effects are reversed antagonist RU38486, proving involvement these actions.ELISA assays demonstrate that impacts on cytokine production shown decrease IFN-c an increase IL-5 production, respectively.Conclusions: Taken together, through their favoring over particular dissociated ligands, for represents paradigm, hold potential application Th1-mediated disorders.