Correction: Matrix Metalloproteinase 13 (MMP13) Is a Direct Target of Osteoblast-Specific Transcription Factor Osterix (Osx) in Osteoblasts

• DOI: 10.1371/annotation/a5ac9734-faa6-41d3-8ba2-6d0894f62db5
• Publication Date: 2013-08-07T00:36:31Z
• Authors: Chi Zhang; Wanjin Tang; Yang Li
• Keywords: 0301 basic medicine; 03 medical and health sciences; Science; Q; R; Medicine; Correction

Osterix (Osx) is an osteoblast-specific transcription factor required for bone formation and osteoblast differentiation from mesenchymal stem cells.In Osx-null mice, no occurs.Matrix metalloproteinase 13 (MMP13) a member of the matrix family plays important role in endochondral ossification remodeling.Transcriptional regulation MMP13 expression osteoblasts not well understood.Here, we provide several lines evidence which show that direct target Osx osteoblasts.Calvaria obtained embryos displayed dramatic reductions compared to wild-type calvaria.Stable overexpression stimulated C2C12 cells.Inhibition by siRNA led downregulation expression.Mechanistic approaches using transient transfection assays showed directly activated 1 kb fragment promoter dose-dependent manner.To define region was responsive Osx, series deletion mutants were examined minimal Osx-responsive refined proximal 80 bp promoter.Additional point mutant analysis used identify one GC-rich responsible activation Osx.Gel Shift Assay bound sequence directly.Chromatin immunoprecipitation demonstrated endogenous associated with native primary vivo.Taken together, these data strongly support regulatory gene osteoblasts.They further new insight into potential mechanisms pathways controls formation.