The €100 lab: A 3D-printable open-source platform for fluorescence microscopy, optogenetics, and accurate temperature control during behaviour of zebrafish, Drosophila, and Caenorhabditis elegans

• DOI: 10.1371/journal.pbio.2002702
• Publication Date: 2017-07-18T13:24:46Z
• Authors: Andre Maia Chagas; Lucia L. Prieto-G...; Aristides B. Arre...; Tom Baden
• Keywords: Codebase

Small, genetically tractable species such as larval zebrafish, Drosophila, or Caenorhabditis elegans have become key model organisms in modern neuroscience. In addition to their low maintenance costs and easy sharing of strains across labs, one appeal is the possibility monitor single groups animals a behavioural arena while controlling activity select neurons using optogenetic thermogenetic tools. However, purchase commercial solution for these types experiments, including an appropriate camera system well controlled arena, can be costly. Here, we present low-cost modular open-source alternative called 'FlyPi'. Our design based on 3D-printed mainframe, Raspberry Pi computer, high-definition Arduino-based optical thermal control circuits. Depending configuration, FlyPi assembled under €100 features optional modules light-emitting diode (LED)-based fluorescence microscopy stimulation Peltier-based temperature stimulator thermogenetics. The complete version with all approximately €200 substantially less if user prepared 'shop around'. All functions through custom-written graphical interface. To demonstrate FlyPi's capabilities, its use series state-of-the-art neurogenetics experiments. addition, utility medical diagnostic tool teaching aid at Neurogenetics courses held several African universities. Taken together, cost nature fully open make it highly versatile range applications, classroom, centres, research labs.