An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold

• DOI: 10.1371/journal.pbio.3002212
• Publication Date: 2023-08-04T17:35:15Z
• Authors: Wenny Wong; Jason A. Estep; Alyssa M. Treptow; Niloofar Rajabli; Jennifer N. Jahncke; Teresa Ubina; Kevin M. Wright; Martin M. Riccomagno
• Keywords: Dystroglycan; Neurite

The mature mammalian cortex is composed of 6 architecturally and functionally distinct layers. Two key steps in the assembly this layered structure are initial establishment glial scaffold subsequent migration postmitotic neurons to their final position. These processes involve precise timely regulation adhesion detachment neural cells from substrates. Although much known about roles adhesive substrates during neuronal formation scaffold, less understood how these signals interpreted integrated within cells. Here, we provide vivo evidence that Cas proteins, a family cytoplasmic adaptors, serve functional redundant role cortical lamination. triple conditional knock-out (Cas TcKO) mice display severe phenotypes feature cobblestone malformations. Molecular epistasis genetic experiments suggest proteins act downstream transmembrane Dystroglycan β1-Integrin radial cell-autonomous manner. Overall, data establish new essential for adaptor circuits reveal signaling axis controlling formation.