Transcriptomic analysis of the 12 major human breast cell types reveals mechanisms of cell and tissue function

• DOI: 10.1371/journal.pbio.3002820
• Publication Date: 2024-11-05T18:33:30Z
• Authors: Katelyn Del Toro; Rosalyn Sayaman; Kate Thi; Yamhilette Licon-...; William Curtis Hines
• Keywords: Cell type; Identification

A fundamental question in biology, central to our understanding of cancer and other pathologies, is determining how different cell types coordinate form maintain tissues. Recognizing the distinct features capabilities cells that compose these tissues critical. Unfortunately, complexity often hinders ability distinguish between neighboring and, turn, scrutinize their transcriptomes generate reliable tractable models for studying inherently biologies. We have recently introduced a novel method permits identification purification 12 human breast—nearly all which could be reliably propagated laboratory. Here, we explore nature types. sequence mRNAs from each purified population investigate transcriptional patterns reveal distinguishing features. describe differentially expressed genes enriched biological pathways capture essence type, highlight transcripts display intriguing expression patterns. These data, analytic tools, analyses rich resource whose exploration provides remarkable insights into inner workings composing breast, thus furthering rules governing normal tissue function.