Statistical analysis of 3D localisation microscopy images for quantification of membrane protein distributions in a platelet clot model

• DOI: 10.1371/journal.pcbi.1007902
• Publication Date: 2020-06-30T20:00:53Z
• Authors: Sandra Mayr; Fabian Hauser; Sujitha Puthukodan; Markus Axmann; Janett Göhring; Jaroslaw Jacak
• Keywords: Multiplex; Statistical Analysis

We present the software platform 2CALM that allows for a comparative analysis of 3D localisation microscopy data representing protein distributions in two biological samples. The in-depth statistical reveals differences between samples at nanoscopic level using parameters such as cluster-density and -curvature. An automatic classification system combines multiplex multi-level approaches into one comprehensive parameter similarity testing compared demonstrated importance 2CALM, comparing CD41 CD62p on activated platelets artificial clot. Additionally, we quantified impact inflammatory cytokine interleukin-1β platelet activation clots. is applicable to any other cell type can provide new insights medical applications.