The Recombinational Anatomy of a Mouse Chromosome

• DOI: 10.1371/journal.pgen.1000119
• Publication Date: 2008-07-10T21:09:25Z
• Authors: Kenneth Paigen; Jin P. Szatkiewicz; Kathryn Sawyer; Nicole Leahy; Emil D. Parvanov; Siemon H. S. Ng; Joel H. Graber; Karl W. Broman; Petko M. Petkov
• Keywords: Ectopic recombination; Chromosomal crossover; Chiasma

Among mammals, genetic recombination occurs at highly delimited sites known as hotspots. They are typically 1-2 kb long and vary much a 1,000-fold or more in activity. Although is about the molecular details of process itself, factors determining location relative activity hotspots poorly understood. To further our understanding, we have collected mapped locations 5,472 crossover events along mouse Chromosome 1 arising 6,028 meioses male female reciprocal F1 hybrids C57BL/6J CAST/EiJ mice. Crossovers were to minimum resolution 225 kb, those telomere-proximal 24.7 Mb resolve individual Recombination rates evolutionarily conserved on regional scale, but not local level. There was clear negative-exponential relationship between abundance hotspot classes, such that small number most active account for majority recombination. Females had 1.2x higher overall than males did, although sex ratio showed considerable variation. Locally, entirely sex-specific rare. The initiation regulated independently two parental chromatids, analysis crosses indicated imprinting has subtle effects rates. It appears regulation mammalian complex, dynamic involving multiple reflecting species, sex, variation within properties