Genome-Wide Transcript Profiling of Endosperm without Paternal Contribution Identifies Parent-of-Origin–Dependent Regulation of AGAMOUS-LIKE36

• DOI: 10.1371/journal.pgen.1001303
• Publication Date: 2011-02-17T19:43:25Z
• Authors: Reza Shirzadi; Ellen D. Andersen; Katrine N. Bjerkan; Barbara M. Gloeckle; Maren Heese; Alexander Ungru; Per Winge; Csaba Koncz; Reidunn B. Aalen; Arp Schnittger; Paul E. Grini
• Keywords: Agamous

Seed development in angiosperms is dependent on the interplay among different transcriptional programs operating embryo, endosperm, and maternally-derived seed coat. In angiosperms, embryo endosperm are products of double fertilization during which two pollen sperm cells fuse with egg cell central female gametophyte. Arabidopsis, analyses mutants cell-cycle regulator CYCLIN DEPENDENT KINASE A;1 (CKDA;1) have revealed importance a paternal genome for effective ultimately seed. Here we exploited cdka;1 as novel tool identification regulators factors involved parent-of-origin–specific regulation development. We generated genome-wide transcription profiles fertilized seeds identified approximately 600 genes that downregulated absence genome. Among those, AGAMOUS-LIKE (AGL) encoding Type-I MADS-box were significantly overrepresented. Here, AGL36 was chosen an in-depth study shown to be imprinted. demonstrate parent-of-origin–dependent expression controlled by activity METHYLTRANSFERASE1 (MET1) maintenance DNA methyltransferase DEMETER (DME) glycosylase. Interestingly, our data also show active maternal allele regulated throughout components FIS Polycomb Repressive Complex 2 (PRC2), revealing new type dual epigenetic seeds.