Phosphorylation-Dependent Differential Regulation of Plant Growth, Cell Death, and Innate Immunity by the Regulatory Receptor-Like Kinase BAK1

• DOI: 10.1371/journal.pgen.1002046
• Publication Date: 2011-04-28T21:11:28Z
• Authors: Benjamin Schwessi...; Milena Roux; Yasuhiro Kadota; Vardis Ntoukakis; Jan Sklenar; Alexandra Jones; Cyril Zipfel
• Keywords: 570; 2716 Genetics (clinical); FLS2 protein; Recombinant Fusion Proteins; protein EFR; protein BRI1; Arabidopsis; 580 Plants (Botany); QH426-470; Protein Serine-Threonine Kinases; protein FLS2; 03 medical and health sciences; 10126 Department of Plant and Microbial Biology; 1311 Genetics; Gene Expression Regulation, Plant; protein serine threonine kinase; 1312 Molecular Biology; Genetics; Point Mutation; 1306 Cancer Research; 10211 Zurich-Basel Plant Science Center; Cloning, Molecular; Phosphorylation; Keywords: protein Bak1; EFR protein; Alleles; BRI1 protein; 0303 health sciences; Arabidopsis protein; Cell Death; Arabidopsis Proteins; protein kinase; hybr; Plants, Genetically Modified; Hypocotyl; Immunity, Innate; unclassified drug; 1105 Ecology, Evolution, Behavior and Systematics; Receptors, Pattern Recognition; BAK1 protein; Steroids; Reactive Oxygen Species; Protein Kinases; Research Article; Signal Transduction

Plants rely heavily on receptor-like kinases (RLKs) for perception and integration of external internal stimuli. The Arabidopsis regulatory leucine-rich repeat RLK (LRR-RLK) BAK1 is involved in steroid hormone responses, innate immunity, cell death control. Here, we describe the differential regulation three different BAK1-dependent signaling pathways by a novel allele BAK1, bak1-5. Innate immune mediated RKs FLS2 EFR severely compromised bak1-5 mutant plants. However, mutants are not impaired BR or We also show that, contrast to RD kinase BRI1, non-RD have very low activity, that neither was able trans-phosphorylate vitro. Furthermore, activity all partners completely dispensable ligand-induced heteromerization with planta, revealing another pathway specific mechanistic difference. suppression FLS2- EFR-dependent due interaction BAK1-5 respective ligand-binding RK but requires activity. Overall our results demonstrate phosphorylation-dependent control plant growth, which may reveal key differences molecular mechanisms underlying RKs.