OSD1 Promotes Meiotic Progression via APC/C Inhibition and Forms a Regulatory Network with TDM and CYCA1;2/TAM

• DOI: 10.1371/journal.pgen.1002865
• Publication Date: 2012-07-27T03:02:24Z
• Authors: Laurence Cromer; Jefri Heyman; Sandra Touati; Hirofumi Harashima; Emilie Araou; Chloe Girard; Christine Horlow; Katja Wassmann; Arp Schnittger; Lieven De Veylder; Raphael Mercier
• Keywords: Meiosis II

Cell cycle control is modified at meiosis compared to mitosis, because two divisions follow a single DNA replication event. Cyclin-dependent kinases (CDKs) promote progression through both and central regulator of their activity the APC/C (Anaphase Promoting Complex/Cyclosome) that especially required for exit from mitosis. We have shown previously OSD1 involved in entry into I II Arabidopsis thaliana; however, molecular mechanism by which controls these transitions has remained unclear. Here we show promotes meiotic inhibition. Next, explored functional relationships between genes known cell Arabidopsis. Like osd1, cyca1;2/tam mutation leads premature after first division, while tdm mutants perform an aberrant third division normal II. Remarkably, epistatic tam, osd1 tdm. further expression non-destructible CYCA1;2/TAM provokes, like tdm, division. Finally, forms active complex with CDKA;1 can phosphorylate vitro. thus propose network composed OSD1, CYCA1;2/TAM, TDM three key steps progression, inhibitor.