Dynamic Distribution of Linker Histone H1.5 in Cellular Differentiation

• DOI: 10.1371/journal.pgen.1002879
• Publication Date: 2012-08-30T17:05:03Z
• Authors: Jing-Yu Li; Michaela Patterson; Hanna K. A. Mikkola; William E. Lowry; Siavash K. Kurdis...
• Keywords: ChIA-PET; Chromodomain; Bivalent chromatin

Linker histones are essential components of chromatin, but the distributions and functions many during cellular differentiation not well understood. Here, we show that H1.5 binds to genic intergenic regions, forming blocks enrichment, in differentiated human cells from all three embryonic germ layers stem cells. In cells, H1.5, H1.3, preferentially genes encode membrane membrane-related proteins. Strikingly, 37% target belong gene family clusters, groups homologous located proximity each other on chromosomes. binding is associated with repression required for SIRT1 binding, H3K9me2 chromatin compaction. Depletion results loss H3K9me2, increased accessibility, deregulation expression, decreased cell growth. Our data reveal first time a specific novel function linker histone subtype maintenance condensed at defined families