A System for Genome-Wide Histone Variant Dynamics In ES Cells Reveals Dynamic MacroH2A2 Replacement at Promoters

• DOI: 10.1371/journal.pgen.1004515
• Publication Date: 2014-08-07T18:07:17Z
• Authors: Ozlem Yildirim; Jui-Hung Hung; Ryan J. Cedeno; Zhiping Weng; Christopher J. Le...; Oliver J. Rando
• Keywords: Epigenomics; Histone code

Dynamic exchange of a subset nucleosomes in vivo plays important roles epigenetic inheritance chromatin states, insulator function, chromosome folding, and the maintenance pluripotent state embryonic stem cells. Here, we extend pulse-chase strategy for carrying out genome-wide measurements histone dynamics to several variants murine cells somatic tissues, recapitulating expected characteristics well characterized H3.3 variant. We extended this system less-studied MacroH2A2 variant, commonly described as "repressive" variant whose accumulation is thought fix differentiated Unexpectedly, found that while large intergenic blocks were stably associated with genome, promoter-associated peaks exhibited relatively rapid ES cells, particularly at highly-transcribed genes. Upon differentiation fibroblasts, was gained primarily additional long, across gene-poor regions, overall turnover promoters greatly dampened. Our results reveal unanticipated dynamic behavior provide resource future studies tissue-specific vivo.