The Chromosomal Association of the Smc5/6 Complex Depends on Cohesion and Predicts the Level of Sister Chromatid Entanglement

• DOI: 10.1371/journal.pgen.1004680
• Publication Date: 2014-10-20T17:15:34Z
• Authors: Kristian Jeppsson; Kristian K. Carlborg; Ryuichiro Nakato; Davide G. Berta; Ingrid Lilienthal; Takaharu Kanno; Arne Lindqvist; Maartje C. Brink; Nico P. Dantuma; Yuki Katou; Katsuhiko Shirahige; Camilla Sjögren
• Keywords: Separase

The cohesin complex, which is essential for sister chromatid cohesion and chromosome segregation, also inhibits resolution of intertwinings (SCIs) by the topoisomerase Top2. cohesin-related Smc5/6 complex (Smc5/6) instead accumulates on chromosomes after Top2 inactivation, known to lead a buildup unresolved SCIs. This suggests that can influence chromosomal association via its role in SCI protection. Using high-resolution ChIP-sequencing, we show localization budding yeast duplicated indeed depends wild-type top2-4 cells. found be enriched at binding sites centromere-proximal regions both cell types, but along arms when replication has occurred under Top2-inhibiting conditions. Reactivation causes dissociate from arms, supporting assumption associates with substrate. It demonstrated amount positively correlates level missegregation top2-4, promotes segregation short mutant. Altogether, this shows predicts presence segregation-inhibiting entities accumulate mutated These are most likely SCIs, our results thus indicate that, least inhibited, facilitates their resolution.