The Global Regulatory Architecture of Transcription during the Caulobacter Cell Cycle

• DOI: 10.1371/journal.pgen.1004831
• Publication Date: 2015-01-08T19:09:37Z
• Authors: Bo Zhou; Jared M. Schrader; Virginia S. Kalog...; Eduardo Abeliuk; Cong B. Dinh; James Q. Pham; Zhongying Z. Cui; David L. Dill; Harley H. McAdams; Lucy Shapiro
• Keywords: Caulobacter crescentus; Transcription

Each Caulobacter cell cycle involves differentiation and an asymmetric division driven by a cyclical regulatory circuit comprised of four transcription factors (TFs) DNA methyltransferase. Using modified global 5′ RACE protocol, we globally mapped start sites (TSSs) at base-pair resolution, measured their levels multiple times in the cycle, identified factor binding sites. Out 2726 TSSs, 586 were shown to be cycle-regulated 529 for master regulators. Twenty-three percent promoters found under combinatorial control two or more Previously unknown features core identified, including 107 antisense TSSs which exhibit cycle-control, 241 genes with whose often exhibited different timing. Cumulatively, this study uncovered novel new layers transcriptional regulation mediating bacterial cycle.