ALIX and ESCRT-III Coordinately Control Cytokinetic Abscission during Germline Stem Cell Division In Vivo

• DOI: 10.1371/journal.pgen.1004904
• Publication Date: 2015-01-30T19:35:06Z
• Authors: Åsmund H. Eikenes; Lene Malerød; Anette Lie Christ...; Chloé B. Steen; Juliette Mathieu; Ioannis P. Nezis; Knut Liestøl; Jean-René Huynh; Harald Stenmark; Kaisa Haglund
• Keywords: Abscission; Midbody; ESCRT

Abscission is the final step of cytokinesis that involves cleavage intercellular bridge connecting two daughter cells. Recent studies have given novel insight into spatiotemporal regulation and molecular mechanisms controlling abscission in cultured yeast human The living metazoan tissues are however not well understood. Here we show ALIX ESCRT-III component Shrub required for completion during Drosophila female germline stem cell (fGSC) division. Loss or function fGSCs leads to delayed consequent formation cysts which chains cells remain interconnected fGSC via midbody rings fusome. We demonstrate interact they co-localize at midbodies cytokinetic fGSCs. Mechanistically, direct interaction between ensure with normal kinetics conclude coordinately control their complex accurate timing GSCs vivo.