Large-Scale Phenomics Identifies Primary and Fine-Tuning Roles for CRKs in Responses Related to Oxidative Stress

• DOI: 10.1371/journal.pgen.1005373
• Publication Date: 2015-07-21T16:09:15Z
• Authors: Gildas Bourdais; Paweł Burdiak; Adrien Gauthier; Lisette Nitsch; Jarkko Salojärvi; Channabasavangowd...; Niina Idänheimo; Kerri Hunter; Sachie Kimura; Ebe Merilo; Aleksia Vaattovaara; Krystyna Oracz; David Kaufholdt; Andres Pallon; Damar Tri Anggoro; Dawid Glów; Jennifer Lowe; Ji Zhou; Omid Mohammadi; Tuomas Puukko; Andreas Albert; Hans Lang; Dieter Ernst; Hannes Kollist; Mikael Brosché; Jörg Durner; Jan Willem Borst; David B. Collinge; Stanisław Karpiński; Michael F. Lyngkjær; Silke Robatzek; Michael Wrzaczek; Jaakko Kangasjärvi
• Keywords: Adapter molecule crk

Cysteine-rich receptor-like kinases (CRKs) are transmembrane proteins characterized by the presence of two domains unknown function 26 (DUF26) in their ectodomain. The CRKs form one largest groups protein plants, but biological functions have so far remained largely uncharacterized. We conducted a large-scale phenotyping approach nearly complete crk T-DNA insertion line collection showing that control important aspects plant development and stress adaptation response to biotic abiotic stimuli non-redundant fashion. In particular, analysis reactive oxygen species (ROS)-related responses, such as regulation stomatal aperture, suggests participate ROS/redox signalling sensing. play general fine-tuning roles closure induced microbial cues. Despite great number high similarity, identified specific diverse processes for many indicated CRK2 CRK5 predominant growth adaptation, respectively. As whole, contribute specificity ROS signalling. Individual distinct responses an antagonistic fashion suggesting future potential using genetic approaches improve performance tolerance.