Redundant Roles of Rpn10 and Rpn13 in Recognition of Ubiquitinated Proteins and Cellular Homeostasis

• DOI: 10.1371/journal.pgen.1005401
• Publication Date: 2015-07-29T18:03:22Z
• Authors: Jun Hamazaki; Shoshiro Hirayama; Shigeo Murata
• Keywords: Ubiquitin-conjugating enzyme

Intracellular proteins tagged with ubiquitin chains are targeted to the 26S proteasome for degradation. The two subunits, Rpn10 and Rpn13, function as receptors of proteasome. However, differences in roles between Rpn13 mammals remains be understood. We analyzed mice deficient Rpn10. Liver-specific deletion either or showed only modest impairment, but simultaneous loss both caused severe liver injury accompanied by massive accumulation conjugates, which was recovered re-expression Rpn13. also found that mHR23B ubiquilin/Plic-1 -4 failed bind absence suggesting these subunits main UBL-UBA deliver ubiquitinated Our results indicate mostly plays a redundant role recognition maintaining homeostasis Mus musculus.