YAP1 Exerts Its Transcriptional Control via TEAD-Mediated Activation of Enhancers

• DOI: 10.1371/journal.pgen.1005465
• Publication Date: 2015-08-21T18:00:12Z
• Authors: Claudia Stein; Anaïs Flore Bardet; Guglielmo Roma; Sebastian Bergling; Ieuan Clay; Alexandra Ruchti; Claudia Agarinis; Tobias Schmelzle; Tewis Bouwmeester; Dirk Schübeler; Andreas Bauer
• Keywords: YAP1; Hippo signaling pathway

YAP1 is a major effector of the Hippo pathway and well-established oncogene. Elevated activity due to mutations in components or amplification observed several types human cancers. Here we investigated its genomic binding landscape YAP1-activated cancer cells, as well non-transformed cells. We demonstrate that TEAD transcription factors mediate chromatin-binding genome-wide, further explaining their dominant role primary mediators YAP1-transcriptional activity. Moreover, show largely exerts transcriptional control via distal enhancers are marked by H3K27 acetylation necessary for this chromatin mark at bound associated genes. This work establishes YAP1-mediated regulation provides an expanded set target genes resulting fundamental source study function normal setting.