The Arabidopsis RCC1 Family Protein TCF1 Regulates Freezing Tolerance and Cold Acclimation through Modulating Lignin Biosynthesis

• DOI: 10.1371/journal.pgen.1005471
• Publication Date: 2015-09-22T21:15:35Z
• Authors: Hongtao Ji; Youning Wang; Catherine Cloix; Kexue Li; Gareth I. Jenkins; Shuangfeng Wang; Zhonglin Shang; Yiting Shi; Shuhua Yang; Xia Li
• Keywords: 0301 basic medicine; Arabidopsis Proteins; Acclimatization; Arabidopsis; Nuclear Proteins; QH426-470; Lignin; Cold Temperature; Histones; 03 medical and health sciences; Gene Expression Regulation, Plant; Gene Knockdown Techniques; Genetics; Carrier Proteins; Research Article

Cell water permeability and cell wall properties are critical to survival of plant cells during freezing, however the underlying molecular mechanisms remain elusive. Here, we report that a specifically cold-induced nuclear protein, Tolerant Chilling Freezing 1 (TCF1), interacts with histones H3 H4 associates chromatin containing target gene, BLUE-COPPER-BINDING PROTEIN (BCB), encoding glycosylphosphatidylinositol-anchored protein regulates lignin biosynthesis. Loss TCF1 function leads reduced BCB transcription through affecting H3K4me2 H3K27me3 levels within resulting in content enhanced freezing tolerance. Furthermore, plants knocked-down expression (amiRNA-BCB) under cold acclimation had accumulation increased The pal1pal2 double mutant (lignin by 30% compared WT) also showed tolerant phenotype, act upstream PALs regulate content. In addition, acts independently CBF (C-repeat binding factor) pathway. Our findings delineate novel pathway linking TCF1-mediated cold-specific transcriptional program biosynthesis, thus achieving remodeling