Loss of a Conserved tRNA Anticodon Modification Perturbs Plant Immunity

• DOI: 10.1371/journal.pgen.1005586
• Publication Date: 2015-10-22T17:57:15Z
• Authors: Vicente Ramírez; Beatriz Gonzalez; Ana López; María José Castelló; María José Gil; Bo Zheng; Peng Chen; Pablo Vera
• Keywords: T arm; Pseudomonas syringae

tRNA is the most highly modified class of RNA species, and modifications are found in tRNAs from all organisms that have been examined. Despite their vastly different chemical structures presence tRNAs, occurring locations tRNA, biosynthetic pathways majority include a methylation step(s). Recent discoveries revealed unprecedented complexity modification patterns regulation function, suggesting each nucleoside may its own specific function. However, plants, our knowledge on role individual how they regulated very limited. In genetic screen designed to identify factors regulating disease resistance activation defenses Arabidopsis, we identified SUPPRESSOR OF CSB3 9 (SCS9). Our results reveal SCS9 encodes methyltransferase mediates 2´-O-ribose selected species anticodon loop. These SCS9-mediated enhance during course infection with bacterial pathogen Pseudomonas syringae DC3000, lack such modification, as observed scs9 mutants, severely compromise plant immunity against same without affecting salicylic acid (SA) signaling pathway which regulates immune responses. support model gives importance control certain for mounting an effective response therefore expands repertoire molecular components essential efficient response.