Genome-Wide and Experimental Resolution of Relative Translation Elongation Speed at Individual Gene Level in Human Cells

• DOI: 10.1371/journal.pgen.1005901
• Publication Date: 2016-02-29T18:31:57Z
• Authors: Xinlei Lian; Jiahui Guo; Wei Gu; Yizhi Cui; Jiayong Zhong; Jingjie Jin; Qing-Yu He; Tong Wang; Gong Zhang
• Keywords: Elongation; Elongation factor; Eukaryotic translation; Ribosome profiling

In the process of translation, ribosomes first assemble on mRNAs (translation initiation) and then translate along mRNA (elongation) to synthesize proteins. Elongation pausing is deemed highly relevant co-translational folding nascent peptides functionality protein products, which positioned evaluation elongation speed as one central questions in field translational control. By integrating three types RNA-seq methods, we experimentally computationally resolved speed, with our proposed velocity index (EVI), a relative measure at individual gene level under physiological condition human cells. We successfully distinguished slow-translating genes from background translatome. demonstrated that low-EVI encoded more stable further identified cell-specific codons, might serve causal factor deceleration. As an example for biological relevance, showed relatively tended be associated maintenance malignant phenotypes per pathway analyses. conclusion, EVI opens new view understand why cells tend avoid simultaneously speeding up translation initiation decelerating elongation, possible cancer relevance translating gain better quality.