Mariner Transposons Contain a Silencer: Possible Role of the Polycomb Repressive Complex 2

• DOI: 10.1371/journal.pgen.1005902
• Publication Date: 2016-03-03T19:05:02Z
• Authors: Solenne Bire; Sophie Casteret; Benoît Piégu; Linda Beauclair; Nathalie Moiré; Peter Arensbuger; Yves Bigot
• Keywords: Silencer; Transposase; Conserved sequence

Transposable elements are driving forces for establishing genetic innovations such as transcriptional regulatory networks in eukaryotic genomes. Here, we describe a silencer situated the last 300 bp of Mos1 transposase open reading frame (ORF) which functions vertebrate and arthropod cells. Functional silencers also found at similar locations within three other animal mariner elements, i.e. IS630-Tc1-mariner (ITm) DD34D Himar1, Hsmar1 Mcmar1. These able to impact promoters monitoring strong, moderate or low expression well those located upstream ORF. We report that silencing involves least two transcription factors (TFs) conserved species, NFAT-5 Alx1. cooperatively act with YY1 trigger activity. Four housekeeping (TFs), neuron restrictive factor (NRSF), GAGA (GAF) GTGT (GTF), were have binding sites but their modulating activity remains be further specified. Interestingly, an NRSF site was overlap 30 motif coding highly PHxxYSPDLAPxD peptide transposases. present experimental evidence is mainly achieved by co-opting host Polycomb Repressive Complex 2 pathway. However, observe when PRC2 impaired another pathway potentially takes over maintain weak Mariner harbour features Response Elements, probably way self-repress mobility somatic germinal cells required TFs expressed. At evolutionary scale, through exaptation, might been source playing role chromatin configuration