Mitochondrial Polyadenylation Is a One-Step Process Required for mRNA Integrity and tRNA Maturation

• DOI: 10.1371/journal.pgen.1006028
• Publication Date: 2016-05-13T17:33:16Z
• Authors: Ana Bratic; Paula Clemente; Javier Calvo-Garrido; Camilla Maffezzini; Andrea Felser; Rolf Wibom; Anna Wedell; Christoph Freyer; Anna Wredenberg
• Keywords: Post-transcriptional modification

Polyadenylation has well characterised roles in RNA turnover and translation a variety of biological systems. While polyadenylation on mitochondrial transcripts been suggested to be two-step process required complete translational stop codons, its involvement is less understood. We studied knockdown knockout models the poly(A) polymerase (MTPAP) Drosophila melanogaster demonstrate that mRNAs exclusively performed by MTPAP. Further, our results show does not regulate mRNA stability but protects 3' terminal integrity, despite lack functioning ends, these trimmed are translated, suggesting for translation. Additionally, loss MTPAP leads reduced steady-state levels disturbed maturation tRNACys, indicating mitochondria might important specific tRNAs.