Nutrient Control of Yeast Gametogenesis Is Mediated by TORC1, PKA and Energy Availability

• DOI: 10.1371/journal.pgen.1006075
• Publication Date: 2016-06-06T17:26:59Z
• Authors: Hilla Weidberg; Fabien Moretto; Gianpiero Spedale; Angelika Amon; Folkert J. van We...
• Keywords: 0301 basic medicine; 2. Zero hunger; 0303 health sciences; Saccharomyces cerevisiae Proteins; Nuclear Proteins; Saccharomyces cerevisiae; QH426-470; Protein Serine-Threonine Kinases; Spores, Fungal; Cyclic AMP-Dependent Protein Kinases; Gametogenesis; DNA-Binding Proteins; Repressor Proteins; Meiosis; 03 medical and health sciences; Genetics; Promoter Regions, Genetic; Research Article; Signal Transduction; Transcription Factors

Cell fate choices are tightly controlled by the interplay between intrinsic and extrinsic signals, gene regulatory networks. In Saccharomyces cerevisiae, decision to enter into gametogenesis or sporulation is dictated mating type nutrient availability. These signals regulate expression of master regulator gametogenesis, IME1. Here we describe how nutrients control IME1 expression. We find that protein kinase A (PKA) target rapamycin complex I (TORC1) signalling mediate regulation Inhibiting both pathways sufficient induce complete in nutrient-rich conditions. Our ability under rich conditions allowed us show respiration fermentation interchangeable energy sources for transcription. Furthermore, TORC1 can promote inhibit gametogenesis. Down-regulation required activate However, inactivation inhibits induction, indicating an intermediate level entry sporulation. Finally, transcriptional repressor Tup1 binds represses promoter when ample, but released from PKA inhibited. Collectively our data demonstrate mediated a combination availability, activities converge on promoter.